| Abstract Detail
Pteridological Section/AFS Schuettpelz, Eric [1], Grusz, Amanda L. [1], Windham, Michael [1], Pryer, Kathleen M. [1]. The utility of nuclear gapCp in resolving polyploid fern origins. Although polyploidy is rampant in ferns and plays a major role in shaping their diversity, the evolutionary history of most polyploid taxa remains unclear. Isozyme electrophoresis can provide important clues regarding polyploid origins; however, low-copy nuclear gene sequencing holds even greater potential as it can be used to place polyploid genomes in a phylogenetic context. Here, we report the development of new primers for the amplification of a portion of the nuclear gapCp gene. Through a broad survey across ferns, we demonstrate that these new primers are nearly universal for this clade. With a case study in cheilanthoids, we show that this rapidly evolving marker is a powerful tool for uncovering polyploid ancestries. Our results indicate that gapCp holds considerable potential for addressing species-level questions across the fern tree of life. Log in to add this item to your schedule
1 - Duke University, Department of Biology, Durham, NC, 27708, USA
Keywords: ferns gapCp GAPDH low-copy nuclear gene molecular systematics nuclear marker polyploidy Pteridaceae species-level phylogenetics.
Presentation Type: Oral Paper:Papers for Sections Session: 23 Location: 209/SUB Date: Monday, July 28th, 2008 Time: 3:00 PM Number: 23005 Abstract ID:514 |